Analysis of chromatin integrity and DNA damage of buffalo spermatozoa

Authors

  • A. A. E. El-Sokary General Organization for Veterinary Services, Dokki, Giza, Egypt
  • A. E. Abdel-Ghaffar Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
  • K. Gh. M. Mahmoud Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt
  • M. E. A. Abou El-Roos Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
  • Y. F. Ahmed Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt
Abstract:

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly significant differences (P

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analysis of chromatin integrity and dna damage of buffalo spermatozoa

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Journal title

volume 16  issue 2

pages  161- 166

publication date 2015-06-20

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